Final
| PL | Athlete | Team | Final | HPL |
|---|
| 1 | Killebrew, Semira | Indiana Storm TC | 26.10 | 4(1) |
| 2 | Hamilton-Ragsda, Sydney | Indiana Storm TC | 27.38 | 4(2) |
| 3 | Robinson, Alyssa | Indys Racing Cheetahs | 28.09 | 1(1) |
| 4 | Covington, Mikeisha | Indiana Storm TC | 28.67 | 4(3) |
| 5 | Green, Gabriele | Indys Racing Cheetahs | 28.69 | 3(1) |
| 6 | Irby, Jo'Deci | Fort Wayne Express TC | 28.83 | 3(2) |
| 7 | Miles, Emonie | Indiana Storm TC | 29.22 | 4(4) |
| 8 | Hollis, ShiDrea | Fort Wayne Express TC | 29.46 | 3(3) |
| 9 | Willis, DaNasha | Indys Racing Cheetahs | 30.06 | 1(2) |
| 10 | Sloan, Carris | Indiana Storm TC | 30.08 | 4(5) |
| 11 | Chestnut, Brooklyn | Indiana Storm TC | 30.27 | 4(6) |
| 12 | Molett, Jaliaya | Indys Racing Cheetahs | 30.39 | 2(1) |
| 13 | Kelley, Bethany | Fort Wayne Express TC | 30.49 | 2(2) |
| 14 | Williams, Joclyn | Indys Racing Cheetahs | 30.51 | 1(3) |
| 15 | Upshaw, Shayla | Indys Racing Cheetahs | 30.66 | 1(4) |
| 16 | Osborn, Imani | Indiana Storm TC | 30.74 | 4(7) |
| 17 | Hunter, Brea | Columbus Running Club | 31.07 | 3(4) |
| 18 | Carr, Sianna | Fort Wayne Express TC | 31.26 | 3(5) |
| 19 | Baxter, Anaiha | Western Boone TC | 31.31 | 3(6) |
| 20 | Sullivan, Zoe | Indys Racing Cheetahs | 31.51 | 1(5) |
| 21 | Johnson, Railei | Indys Racing Cheetahs | 31.97 | 3(7) |
| 22 | Manuel, Kiersten | Indys Racing Cheetahs | 32.71 | 2(3) |
| 23 | Rice, Anetta | Indys Racing Cheetahs | 33.04 | 2(4) |
| 24 | Prince, Grace | Indys Racing Cheetahs | 33.26 | 2(5) |
| Webster, Laniya | Indys Racing Cheetahs | DNS | 1() |
| McGuire, Olivia | Unattached | DNS | 2() |
Final Heat: 1
| PL | HPL | Athlete | Team | Final |
|---|
| 3 | 1 | Robinson, Alyssa | Indys Racing Cheetahs | 28.09 |
| 9 | 2 | Willis, DaNasha | Indys Racing Cheetahs | 30.06 |
| 14 | 3 | Williams, Joclyn | Indys Racing Cheetahs | 30.51 |
| 15 | 4 | Upshaw, Shayla | Indys Racing Cheetahs | 30.66 |
| 20 | 5 | Sullivan, Zoe | Indys Racing Cheetahs | 31.51 |
| | Webster, Laniya | Indys Racing Cheetahs | DNS |
Final Heat: 2
| PL | HPL | Athlete | Team | Final |
|---|
| 12 | 1 | Molett, Jaliaya | Indys Racing Cheetahs | 30.39 |
| 13 | 2 | Kelley, Bethany | Fort Wayne Express TC | 30.49 |
| 22 | 3 | Manuel, Kiersten | Indys Racing Cheetahs | 32.71 |
| 23 | 4 | Rice, Anetta | Indys Racing Cheetahs | 33.04 |
| 24 | 5 | Prince, Grace | Indys Racing Cheetahs | 33.26 |
| | McGuire, Olivia | Unattached | DNS |
Final Heat: 3
| PL | HPL | Athlete | Team | Final |
|---|
| 5 | 1 | Green, Gabriele | Indys Racing Cheetahs | 28.69 |
| 6 | 2 | Irby, Jo'Deci | Fort Wayne Express TC | 28.83 |
| 8 | 3 | Hollis, ShiDrea | Fort Wayne Express TC | 29.46 |
| 17 | 4 | Hunter, Brea | Columbus Running Club | 31.07 |
| 18 | 5 | Carr, Sianna | Fort Wayne Express TC | 31.26 |
| 19 | 6 | Baxter, Anaiha | Western Boone TC | 31.31 |
| 21 | 7 | Johnson, Railei | Indys Racing Cheetahs | 31.97 |
Final Heat: 4
| PL | HPL | Athlete | Team | Final |
|---|
| 1 | 1 | Killebrew, Semira | Indiana Storm TC | 26.10 |
| 2 | 2 | Hamilton-Ragsda, Sydney | Indiana Storm TC | 27.38 |
| 4 | 3 | Covington, Mikeisha | Indiana Storm TC | 28.67 |
| 7 | 4 | Miles, Emonie | Indiana Storm TC | 29.22 |
| 10 | 5 | Sloan, Carris | Indiana Storm TC | 30.08 |
| 11 | 6 | Chestnut, Brooklyn | Indiana Storm TC | 30.27 |
| 16 | 7 | Osborn, Imani | Indiana Storm TC | 30.74 |